碳酸酐酶1沉默对肺癌A549细胞增殖、凋亡、侵袭和迁移的影响

目的:探讨沉默碳酸酐酶1（CA1）对人肺癌A549细胞增殖、凋亡、侵袭和迁移的影响。方法:采用脂质体转染法将CA1特异性siRNA（si-CA1组）及阴性对照（si-NC组）转染肺癌A549细胞，同时以转染空脂质体的A549细胞为空白对照组（Blank组）。采用实时荧光定量PCR（qPCR）和蛋白印迹法（Western blot）检测CA1 mRNA和蛋白表达水平，细胞计数试剂盒法（CCK-8）、流式细胞术、Transwell实验分别检测A549细胞增殖、凋亡、侵袭和迁移能力。结果:qPCR和Western blot检测结果显示，转入CA1 siRNA的A549细胞中CA1 mRNA和蛋白的表达水平均明显下调（P<0.05）；CCK-8实验结果显示，沉默CA1后si-CA1组A549细胞在24、48和72 h时光密度（OD）值明显低于si-NC组（P<0.05）；流式细胞术结果发现，si-CA1组A549细胞凋亡率明显高于si-NC组（P<0.05）；Transwell实验结果显示，si-CA1组侵袭和迁移细胞数均明显少于si-NC组（P<0.05）。与Blank组相比，si-NC组以上各指标差异均不显著（P>0.05）。结论:沉默CA1能够抑制肺癌A549细胞增殖、侵袭和迁移，并促进细胞凋亡。     

Synthesis and Cell Growth Inhibitory Activity of Six Non-glycosaminoglycan-Type Heparin-Analogue Trisaccharides

The design and synthesis of heparin mimetics with high anticancer activity but no anticoagulant activity is an important task in medicinal chemistry. Herein, we present the efficient synthesis of five Glc-GlcA-Glc-sequenced and one Glc-IdoA-Glc-sequenced non-glycosaminoglycan, heparin-related trisaccharides with various sulfation/sulfonylation and methylation patterns. The cell growth inhibitory effects of the compounds were tested against four cancerous human cell lines and two non-cancerous cell lines. Two d -glucuronate-containing tetra-O-sulfated, partially methylated trisaccharides displayed remarkable and selective inhibitory effects on the growth of ovary carcinoma (A2780) and melanoma (WM35) cells. Methyl substituents on the glucuronide unit proved to be detrimental, whereas acetyl substituents were beneficial to the cytostatic activity of the sulfated derivatives.     

Quantification and source characterization of volatile organic compounds from exercising and application of chlorine-based cleaning products in a university athletic center

Humans spend approximately 90% of their time indoors, impacting their own air quality through occupancy and activities. Human VOC emissions indoors from exercise are still relatively uncertain, and questions remain about emissions from chlorine-based cleaners. To investigate these and other issues, the ATHLETic center study of Indoor Chemistry (ATHLETIC) campaign was conducted in the weight room of the Dal Ward Athletic Center at the University of Colorado Boulder. Using a Vocus Proton-Transfer-Reaction Time-of-Flight Mass Spectrometer (Vocus PTR-TOF), an Aerodyne Gas Chromatograph (GC), an Iodide-Chemical Ionization Time-of-Flight Mass Spectrometer (I-CIMS), and Picarro cavity ringdown spectrometers, we alternated measurements between the weight room and supply air, allowing for determination of VOC, NH₃, H₂O, and CO₂ emission rates per person (emission factors). Human-derived emission factors were higher than previous studies of measuring indoor air quality in rooms with individuals at rest and correlated with increased CO₂ emission factors. Emission factors from personal care products (PCPs) were consistent with previous studies and typically decreased throughout the day. In addition, N-chloraldimines were observed in the gas phase after the exercise equipment was cleaned with a dichlor solution. The chloraldimines likely originated from reactions of free amino acids with HOCl on gym surfaces.     

Indoor emissions of total and fluorescent supermicron particles during HOMEChem

Inhalation of particulate matter is associated with adverse health outcomes. The fluorescent portion of supermicron particulate matter has been used as a proxy for bioaerosols. The sources and emission rates of fluorescent particles in residential environments are not well-understood. Using an ultraviolet aerodynamic particle sizer (UVAPS), emissions of total and fluorescent supermicron particles from common human activities were investigated during the HOMEChem campaign, a test-house investigation of the chemistry of indoor environments. Human occupancy and activities, including cooking and mopping, were found to be considerable sources of indoor supermicron fluorescent particles, which enhanced the indoor particle concentrations by two orders of magnitude above baseline levels. The estimated total (fluorescent) mass emission rates for the activities tested were in the range of 4-30 (1-11) mg per person meal for cooking and 0.1-4.9 (0.05-4.7) mg/h for occupancy and mopping. Model calculations indicate that, once released, the dominant fate of coarse particles (2.5-10 micrometer in diameter) was deposition onto indoor surfaces, allowing for the possibility of subsequent resuspension and consequent exposures over durations much longer than the ventilation time scale. Indoor coarse particle deposition would also contribute to soiling of indoor surfaces.     

Secretory immunoglobulin A from human milk hydrolyzes 5 histones and myelin basic protein

Mother's milk provides newborns with various nutrients (e.g., enzymes, proteins, peptides, hormones, antibodies) that help babies grow and protect them from bacterial and viral infections. The functions of many components of breast milk can be very different from their corresponding functions in body fluids of healthy adults. Catalytic antibodies (abzymes) that hydrolyze peptides, proteins, DNA, RNA, and oligosaccharides were detected not only in human milk, but also in the blood sera of autoimmune patients. However, abzymes with unexpected synthetic activities (lipids, oligosaccharides, and protein kinase activities) were revealed in milk that were not found in the blood of autoimmune patients. The nutrition of infants with fresh milk has a very specific role; newborns are well protected by antibodies of mother's milk (passive immunity). Protease abzymes were found in the blood sera of autoimmune patients, whereas healthy humans usually do not contain such autoantibodies. Here, we present the first evidence that the milk of healthy mothers contains secretory (s)IgA that effectively hydrolyze 5 histones (e.g., H1, H2A, H2B, H3, and H4) and myelin basic protein (MBP). Several rigid criteria were applied to show that protease activity is an intrinsic property of sIgA. Milk abzymes against 5 histones cannot hydrolyze different control proteins except histones and MBP, whereas autoantibodies against MBP split this protein and 5 histones. Antibodies against histones and MBP exhibit complexation polyreactivity as well as specific and unusual catalytic cross-reactivity. With some exceptions, the specific sites of hydrolysis of H1, H2A, and H2B by sIgA against histones do not coincide with the sites of hydrolysis by abzymes against MBP. On the whole, fresh human milk is a very specific source of many of the most unusual antibodies and abzymes.     

城市湿地对城市人居环境可持续发展影响机制初探

界定了城市湿地与城市人居环境的概念,分析了城市人居环境可持续发展的内涵,通过研究城市湿地对城市人居环境可持续发展的影响机制和模式、影响途径与方式以及影响的领域,提出随着城市湿地的类型、分布状况与数量配置的差异,服务功能的健康与否,湿地多样性的不同,将对城市人居环境系统的住区系统、人工支撑系统、生态环境系统、社会经济系统和城市精神文化系统造成不同程度的影响。旨在为城市人居环境建设及其与城市湿地的协调发展提供理论借鉴。     

资源型城市避免资源诅咒的根本在于人力资源开发

世界经济的发展表明,如果不能适当利用自然资源,则丰富的自然资源对于一个城市或地区来说不是福而是祸。资源型城市如何避免资源诅咒,成功转型,最关键的是开发人力资源,从依赖自然资源走向依靠人力资源的道路上来。     

浅谈我国国有矿山企业人力资源管理

21世纪人力资源管理在企业中的地位日趋重要。通过对矿业人力资源管理的现状分析，结合当前我国国有企业发展理论、市场经济发展理论，对我国矿山企业人力资源管理的改革进行了探讨，以期对我国国民经济的发展有所参考。     

企业青年人力资源开发重在培养创新型人才

对企业在青年人力资源开发工作中,创新型人才表现出来的特征以及如何培养创新型人才进行了阐述,为企业青年人力资源开发工作起到一定的指导作用。     

论激励理论在企业中的应用

企业最大的资本是拥有一批没有发挥潜力的员工,挖掘员工的潜力,充分调动员工的积极性是企业建立激励机制的目的.着重对物质激励、精神激励以及情感激励进行了详细的阐述,并描述了每种激励方式下的员工的行为表现.希望能够对企业建立有效的激励机制带来帮助.